报告题目： Australian Research Council Centre of Excellence for Nanoscale Biophotonics - Fundamental Science and Applied Technologies
报 告 人：Professor Ewa M. Goldys，Macquarie University
报告时间：2016年11月3日（星期四）下午4：00
报告地点：化学楼五楼学术报告厅
Australian Research Council Centre of Excellence for Nanoscale Biophotonics - Fundamental Science and Applied Technologies
While an understanding of many of the building blocks and processes of life has been forged by taking cells and systems outside the body, much more can be learnt from working within. The Centre of Excellence in Nanoscale BioPhotonics (CNBP) will perform the science required to open new windows into the body. This interdisciplinary program aims to enable targeted measurements of biological, chemical and physical processes to be performed within the complex and dynamic microenvironment that cells experience, creating scientific insights and disruptive technologies.
One of the key technologies developed by the Centre is fluorescence, which has now become one of the key detection methods in genomics, proteomics, cell biology and biomedical diagnostics. Ultrasensitive fluorescence detection strategy requires bright and stable bioprobes that have high absorption coefficients and high quantum yields and excellent signal to background ratio. Nanoparticles are especially attractive because of their capacity for molecular targeting. In the first part of this talk I will describe our achievements in the development of nanoparticle bioprobes for providing these features. Several strategies were used to improve the detection sensitivity, including time-gating, increasing the density of emitters (nanoruby, upconverting nanoparticles), and modification of luminescence properties by plasmonic amplification (Ag@SiO2nanoparticles with core-shell geometry).
Further, I will discuss our advances in extracting information from endogenous fluorophores in biological systems. Biological cells are very heterogeneous and significant major subpopulations have been uncovered in stem cells, neurons, cancer, immune cells and many other cell types. The understanding of these biochemically and functionally different cell subpopulations will revolutionise biology and medicine. We developed of specialised characterization hardware and analysis tools using autofluorescence hyperspectral imaging able to identify and help select cell populations with different biochemistry, without biochemical interference with these cells. These methods have been applied to a number of cell types including olfactory neuronal cells, adipose-derived stem cells, induced pluripotent stem (IPS) cells, motor neurone disease cells, various cancer cells, embryo and diabetic tissue. We explain how our method responds to commercial and clinical needs across a broad spectrum of medicine and the life sciences.
About the speaker
Professor Ewa M. Goldys Deputy Director of the Australian Research Council Centre of Excellence for Nanoscale Biophotonics at Macquarie University Syndey, Australia, jointly set up with the University of Adelaide and RMIT (see www.cnbp.org.au).
Professor Goldys attractedover $60,000,000 in external competitive research funding, also for interdisciplinary research. She has also been a long-term International Member of Photonics4Life, a key European consortium in Biophotonics.
Professor Goldys pioneered ultrasensitive analysis methods of non-invasive label-free characterisation for biology and health diagnostics. This characterisation is based upon the detection and quantification of various metabolites (free and bound NADH, flavins, A2E, lipofuscin, and cytochrome C). This patented approach provides a collective metabolic fingerprint” which can be used to distinguish healthy from diseased cells in a variety of disease conditions.
Professor Goldys made major contributions to fluorescent labelling, a key optical technique to characterise cells and tissues by characterising and developing applications of specialised fluorescent nanoparticles including the first successful demonstration of fluorescence upconversion in nanoparticles (2006) and a sequence of works concerned with optical characterisation of lanthanide-doped nanoparticles, including publications in Nature Nanotechnology (2013) and Nature Photonics (2014).
Professor Goldys is Fellow Optical Society (2010). The citation reads "For research leadership in optical characterisation and biomedical sensing that has promoted widespread interdisciplinary awareness of light in life sciences”. She received the prestigious Australian Museum 2016 ANSO Eureka Prize for Innovative Use of Technology.
